Advances in Chromatography & HPLC Techniques

Separation

A separation is a method which is used to achieve any phenomenon that converts a mixture of chemical substance into two or more distinct product mixtures, which may be referred to as mixture on which at least one of which is enriched in one or more of the mixture's constituents. Material mixtures separation processes are used to decrease the complexity of mixtures. Some of the representatives of this field are Chromatography, Electrophoresis and Field Flow Fractionation. Majorly between the constituents of a mixture, separations differ in chemical properties or physical properties such as size, shape, mass, density, or chemical affinity. They are classified particularly based on the particular differences they are using to achieve separation. Generally there is only physical movement and no substantial chemical modifications. If no single difference can be used to accomplish a desired separation, multiple operations will often be performed in combination to achieve the desired end.