Principles and Applications of HPLC
HPLC/UHPLC chromatography is a commonly used separation mode in Reversed phase. Its retention of compounds possessing hydrophobic and organic functionality are provided dynamically. Combination of both hydrophobic and van der Waals type interactions between all the target compounds including both the stationary and mobile phases enables by reversed phase retention of these compounds.
HPLC can be used in the following applications:
- Purification of Water
- Pre-concentration for components of trace amount
- Ligand-exchange chromatography
- For Proteins, Ion-exchange chromatography
- For carbohydrates and oligosaccharides, High-pH anion-exchange chromatography
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