Advances in Chromatography & HPLC Techniques

Principle and Applications of Low pressure liquid chromatography

Chromatography systems are often defined by their pressure characteristics. Common acronyms like LP or HPLC are used to refer to low pressure and high-pressure liquid chromatography respectively. Systems that operate at pressures <50 psi (~3 bar) are categorized as low-pressure chromatography systems. Low pressure chromatography is often used for simple protein separations that do not require high resolution. Low pressure systems range from basic to semi-automated and can be equipped with gradient capabilities, detectors, valves, and fraction collectors.

Applications:

• Low pressure systems are ideal for the purification of recombinant tagged proteins and work with most pre-packed column.
• Low pressure systems requires a sample pump and are often equipped with fraction collectors, gradient capabilities and detectors to monitor