18th International Conference on World HPLC & Separation Techniques August 29-30, 2018 Toronto, Ontario, Canada

Biography:

Abstract:

Biography:

David M. Parish Staff Scientist in Protective & Marine Division at Sherwin Williams Company Staff Scientist at Glatfelter, Chilicothe, OH. Sean Zuckerman, PhD (2013): Case Western Reserve University and Nivasu Venkata Muram, PhD (2012). Ohio State University – BS (Organic Chemistry), 1986 Collaborators & Other Affiliations- Horst von Recum, PhD (Biomedical Engineering, Case); Patrick Ziemer (Corporate Polymers Group, Sherwin Williams (SHW)); Andrew Taylor, PhD (Lead Scientist-UK, SHW); Petra Allef, PhD (Innovation, Evonik); Thomas Klotzbach, PhD (Senior Lab Manager-Additives & Silicone Resins, Evonik); Gerald L. Witucki, (Assoc. Scientist, DOW Corning); Maria Nargiello, PhD, (Technical Director, Evonik); Jeffery A. Klang, PhD (R&D Manager, Sartomer Corp.); Leo J. Procopio, PhD (Group Leader-Industrial Coatings, DOW); Seth T. Taylor, PhD (Senior Materials Engineer, Chevron); Jacque Pointcloux, PhD ( Technical Manager, Huntsman Corp.); Ray Drumwright, PhD (Research Fellow, DOW); Dean Webster, PhD (Coatings Science &Technology, Dean, NDSU); William D. Coggio, PhD (Bio-derived Raw Materials, Bio Amber, Inc.)

Abstract:

Academia has always used analytical techniques to characterize, test and further promote the outcome of their respective research. Industry, on the other hand, has primarily utilized analytical science as a forensic tool to help solve product and/or process issues. Each endeavor has merit and definitely is needed, especially in their respective genre. Each physical laboratory is constructed with these types of needs in mind. The Academic laboratory usually contains the equipment required to perform these analytical functions, since it is so central to their project work. Whereas, most industrial laboratories have a completely separated analytical department/lab area due to the fact that their major function is in support of the sales and/or manufacturing arm of the company. These two endeavors need to be better aligned, such that industry can learn from Academia the importance of analytical sciences to build robust formulations/products during the development phase in order to eliminate the potential for problems after product launch. This will also allow for the building of a better understanding of the structure/ property relationship.

Biography:

Abuzar Kabir, a Research Assistant Professor at the International Forensic Research Institute (IFRI), Department of Chemistry and Biochemistry, Florida International University (FIU), Miami, Florida, USA, is a Separation Scientist and Materials Chemist. He has received his Ph.D. in analytical chemistry from University of South Florida (USF), Tampa, Florida, USA with specialization in sol-gel synthesis. He has invented 16-patented technologies in the area of chromatographic separation and analytical/bioanalytical sample preparation. He has also authored/co-authored 9 book chapters, 6 review articles, 46 research articles and 89 conference papers.

Abstract:

Metabolomics plays an important role in discovering potential disease biomarkers from blood plasma or serum samples. Due to the distinctive complexity of whole blood as the sample matrix, either plasma or serum are used as the primary sample in metabolomics biomarker discovery research. During the transformation of whole blood into plasma or serum followed by extraction of targeted or non-targeted metabolites using conventional sample preparation techniques including solid phase extraction (SPE) and liquid-liquid extraction (LLE), a significant portion of the analytical information disappears, resulting in negligible success in discovering potential disease biomarkers. Fabric phase sorptive extraction (FPSE), a new generation sample preparation technology, has offered a paradigm shift approach in metabolomics sample preparation. FPSE innovatively combines the benefits of solid phase extraction (SPE) (works under exhaustive extraction principle) and solid phase microextraction (works under equilibrium extraction principle) into a single sample preparation technology platform. FPSE utilizes a flexible and permeable fabric substrate, coated with high-performance sol-gel sorbents as the extraction media. This uniquely designed extraction medium is capable of extracting target analyte(s) directly from whole blood. Due to the special geometry of FPSE medium (flexible, flat and permeable) and sponge-like porous architecture of sol-gel sorbents, rapid analyte mass transfer occurs between the bulk sample and the extraction medium, resulting in a near-exhaustive extraction within a fraction of time required for other comparable sample preparation techniques. FPSE is particularly suitable for analyzing target analytes e.g., metabolites, biomarkers directly from whole blood without requiring any protein precipitation or other pre-extraction sample cleaning/manipulation. After extracting the target analyte(s) directly from the whole blood sample, FPSE media is exposed to a small volume of organic/organo-aqueous solvent for eluting the extracted analyte(s). The low viscosity of the organic solvent, the capillary force of the fabric support and sponge-like porous sol-gel network allows fast diffusion of organic solvent into the FPSE medium for quick and complete recovery of the extracted analyte(s). As a result, FPSE completely eliminates time-consuming and error-prone solvent evaporation and sample reconstitution step often considered as an integral part of solid phase extraction/liquid-liquid e work-flow. During the solvent-mediated elution/back-extraction, any protein or matrix interferents adhered to the FPSE medium precipitates out and a final centrifugation of the resulting solution prior to injecting into the analytical instrument ensures clean particle-free highly concentrated target analyte(s). Fabric phase sorptive extraction has already developed a large number of sol-gel sorbents specifically suitable for polar metabolites/biomarkers such as sol-gel polyethylene glycol, sol-gel chitosan, sol-gel Carbowax 20M, sol-gel polycaprolactone-dimethylsiloxane-caprolactone to name a few. These high-efficiency sorbents have been found equally effective for analytes with a wide range of polarity. As a consequence, searching for a new disease biomarker from whole blood in presence of numerous endogenous and exogenous interferents is no longer a wishful thinking but an achievable reality. In the current talk, some new and fascinating data on metabolomics sample preparation using FPSE and a comparison between FPSE and conventional sample preparation techniques will be presented.

Biography:

Rob O'Brien, Ph.D., President and Chief Technology Officer, was a professor in Analytical Chemistry for over 13 years and has more than 25 years of experience in analytical chemistry. An expert in analytical instrumentation, he has set up a number of advanced analytical laboratories and has held an executive position in a number of commercial enterprises. Rob O'Brien possesses a track record of successful commercialization of intellectual property developed from academic research. During his academic career, Rob O'Brien secured over 3 million dollars in research grants and has developed an extensive network of research collaborators.

Abstract:

The imminent legal Cannabis Sector is projected to be worth 6 to 20 billion dollars in Canada alone. The highly regulated nature of this sector demands rigorous quality control measures that require advanced analytical mass spectrometry and other techniques to ensure product safety. The nature of the Cannabis plant and its current status as an illegal and controlled narcotic in many countries, add a level of complexity to the development of analytical protocols. This talk will highlight some of the challenges facing this sector and some of the approaches to overcome these. Specifically, issues with potency testing, quantifying medicinal dose delivery, pesticide testing, development of Certified Reference Materials and the importance of ISO 17025 accreditation and international standard development will all be discussed.